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Repositorio Institucional Continental

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Title: Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom
Authors: Torres-Huaco, Frank Denis
Marukñak, Silvana
Teibler, Pamela
Bustillo, Soledad
Acosta de Pérez, Ofelia
Leiva, Laura Cristiina
Ponce-Soto, Luis Alberto
Marangoni, Sergio
Keywords: Aminoácidos
Elementos tóxicos
Enfermedades pulmonares
Publisher: Universidad Continental
Issue Date: 1-Dec-2019
Date available: 10-Jul-2020
Bibliographic citation: Torres-Huaco, F.D., Maruñak, S., Teibler, P. et al. Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom. International Journal of Biological Macromolecules. 141(1), 1044-1054, (2019).
Abstract: A new weak hemorrhagic metalloproteinase named BtaMP-1 was purified from Bothriopsis taeniata snake venom by molecular exclusion followed by anion exchange chromatographies. This protein showed a molecular mass of 25,968.16 Da and is composed of 218 amino acid residues. The multiple alignments of its partial amino acid sequence showed high structural identity with other P-I class SVMP. BtaMP-1 showed caseinolytic activity that was enhanced by Ca2+ ion, completely inhibited by chelating and reducing agents and can be classified as an α-fibrinogenolytic enzyme. Locally, BtaMP-1 induces hemorrhage and edema, but not myotoxicity. These findings were confirmed by histological analysis of mouse gastrocnemius muscle. “In vitro” studies suggest that BtaMP-1 induce cytotoxicity in myoblast C2C12 but not in the myotubes cell line. BtaMP-1 induced systemic alterations in mice with one MHD and two hours exposure; histological analysis of lungs showed hemorrhagic areas, congestion, and increase the thickness of alveolar septum. Also, this protein induced mild effects on kidney and disruption of coagulation by depletion of fibrinogen plasma levels. This work provides insights into the importance of BtaMP-1 biological effects in envenomation by Bothropsis taeniata snake venom and providing further evidence to understand the role of P-I class SVMP in ophidian envenomation.
Notes: Aminoácidos, elementos tóxicos, enfermedades pulmonares
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metadata.dc.format.extent: p. 1044-1054
Access: Restringido
Source: Universidad Continental
Repositorio Institucional - Continental
Appears in Collections:Artículos Científicos

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