Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorGutarra Castillo, Boris-
dc.contributor.authorDíez-Municio, Marina-
dc.contributor.authorCanchaya Garcia, Susan-
dc.contributor.authorCalixto Zacarias, Gaby-
dc.contributor.authorCisneros de la Cruz, Juan-
dc.contributor.authorMoreno, F. Javier-
dc.identifier.citationGutarra, B., Díez, M., Canchaya. S., Calixto, G., Cisneros, J., Moreno. (2018). Diversity of Aspergillus isolates and selection of an isolate with high β-fructofuranosidase activity that is native to the Peruvian coast. African Journal of Biotechnology, 1(1), 5-10.
dc.description.abstractIn order to obtain a native isolate with high β-fructofuranosidase activity (FFase), a preliminary selection of 44 isolates, predominantly formed by Aspergillus niger (72%), was performed among 167 isolates of Aspergillus native to the Peruvian coast (Casa grande, Cartavio, Paramonga and Tacama). In addition, genetic diversity was studied using molecular markers, Inter Simple Sequence Repeats (ISSR). The FFase activity of these 44 isolates was compared and the isolate which showed the highest fructosyltransferase (FTase) activity was identified molecularly at the species level by DNA sequencing. Cluster analysis indicated 16 sub-genetic groups of which 11 of them were morphologically identified as Aspergillus niger. Samples from Paramonga showed the highest genetic diversity as explained by the Shannon diversity index (I= 0.21). No positive association was found between the genetic diversity of populations and FFase activity. Isolates PR-151, PR-144 and PR-142 showed the highest FFase activity in 5 consecutive evaluation generations, PR-142 being the most active with a total FFase activity of 11,248 (U.L-1) and 6.17 g/L of biomass indicating a great biotechnological potential for the synthesis of prebiotics. The molecular identification at the species level confirmed that PR-142 belongs to A. niger lineage.es_ES
dc.format.extentp. 1221-1229es_ES
dc.publisherUniversidad Continentales_ES
dc.sourceUniversidad Continentales_ES
dc.sourceRepositorio Institucional - Continentales_ES
dc.titleDiversity of Aspergillus isolates and selection of an isolate with high β-fructofuranosidase activity that is native to the Peruvian coastes_ES
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)es_ES
dc.rights.accessRightsAcceso abiertoes_ES
dc.identifier.journalAfrican Journal of Biotechnologyes_ES
Appears in Collections:Artículos Científicos

Files in This Item:
There are no files associated with this item.

This item is licensed under a Creative Commons License Creative Commons